- Title
- Sequential changes in expression of long non-coding RNAs THRIL and MALAT1 after ischemic stroke
- Creator
- Bayat, Mahnaz; Hooshmandi, Etrat; Spratt, Neil; Levi, Christopher; Borhani-Haghighi, Afshin; Karimi, Najmeh; Rahimi, Moosa; Tabrizi, Reza; Asadabadi, Tahereh; Salehi, Mohammad Saied; Zafarmand, Seyedeh Shaghayegh; Owjfard, Maryam; Esperon, Carlos Garcia
- Relation
- Current Journal of Neurology Vol. 23, Issue 1, p. 74-82
- Publisher Link
- http://dx.doi.org/10.18502/cjn.v23i1.16437
- Publisher
- Tehran University of Medical Sciences
- Resource Type
- journal article
- Date
- 2024
- Description
- Background: Inflammation is the major contributor to the pathophysiology of ischemic stroke (IS). Long non-coding ribonucleic acids (lncRNAs) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and tumor necrosis factor and heterogeneous nuclear ribonucleoprotein L-related immunoregulatory (THRIL) have been demonstrated to be up-regulated in inflammation and atherosclerosis. Therefore, we aimed to study the expression profile of these lncRNAs after IS. Methods: This observational case-control study was conducted in Namazi Hospital, Shiraz, Iran. The real-time polymerase chain reaction (RT-PCR) measured the sequential changes in circulating levels of MALAT1 and THRIL on days 1, 3, and 5 after IS. The receiver operating characteristic (ROC) curve analysis was used to estimate the diagnostic and prognostic potential of lncRNAs with the area under the curve (AUC). Results: In patients with IS, the relative MALAT1 and THRIL expressions were significantly higher than the controls (P < 0.001 and P < 0.01, respectively), on days 1, 3, and 5 after stroke. We showed a significantly increase in lncRNAs expression on day five compared to days 1 and 3 after stroke. Moreover, a positive correlation was detected between MALAT1 expression and time within the first 24 hours after stroke (r = 0.27, P = 0.03). Logistic regression analysis showed a significant positive association between MALAT1 and THRIL and the risk of stroke evolution. We found a potential diagnostic marker for MALAT1 with an AUC of 0.78. Conclusion: We demonstrated the significant sequential upregulation in MALAT1 and THRIL expression on days 1, 3, and 5 after IS with a significant positive association with the risk of stroke. MALAT1 also significantly correlated with time within the first 24 hours after stroke.
- Subject
- long noncoding RNA; MALAT1 long noncoding RNA; THRIL long noncoding RNA; ischemic stroke; SDG 3; SDG 17; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1517997
- Identifier
- uon:57208
- Identifier
- ISSN:2717-011X
- Rights
- x
- Language
- eng
- Reviewed
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